Construct: shRNA TRCN0000037694

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014216.3-697s1c1
DNA Barcode:
CGGCTTGACTTTCCCATTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITPK1 (3705)
Gene Description:
inositol-tetrakisphosphate 1-kinase
Transcript:
RefSeq NM_014216.3 (NON-CURRENT)
Match location:
Position 697 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037694)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTTGACTTTCCCATTCAT
Hairpin Sequence:
5'-CCGG-CGGCTTGACTTTCCCATTCAT-CTCGAG-ATGAATGGGAAAGTCAAGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTTGACTTTCCCATTCATCTCGAGATGAATGGGAAAGTCAAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTTGACTTTCCCATTCATCTCGAGATGAATGGGAAAGTCAAGCCG-3'

Other clones with same target sequence:

  1. TRCN0000333659