Construct: shRNA TRCN0000037742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025211.2-524s1c1
DNA Barcode:
GTCTCAAACTTTAGGAAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GKAP1 (80318)
Gene Description:
G kinase anchoring protein 1
Transcript:
RefSeq NM_025211.2 (NON-CURRENT)
Match location:
Position 524 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTCAAACTTTAGGAAGCAA
Hairpin Sequence:
5'-CCGG-GTCTCAAACTTTAGGAAGCAA-CTCGAG-TTGCTTCCTAAAGTTTGAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTCAAACTTTAGGAAGCAACTCGAGTTGCTTCCTAAAGTTTGAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTCAAACTTTAGGAAGCAACTCGAGTTGCTTCCTAAAGTTTGAGAC-3'

Other clones with same target sequence:

(none)