Construct: shRNA TRCN0000037746

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002513.2-414s1c1
DNA Barcode:
GCCTTGTCAAGTATATGGCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME3 (4832)
Gene Description:
NME/NM23 nucleoside diphosphate kinase 3
Transcript:
RefSeq NM_002513.2 (CURRENT)
Match location:
Position 414 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037746)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGTCAAGTATATGGCCT
Hairpin Sequence:
5'-CCGG-GCCTTGTCAAGTATATGGCCT-CTCGAG-AGGCCATATACTTGACAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGTCAAGTATATGGCCTCTCGAGAGGCCATATACTTGACAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGTCAAGTATATGGCCTCTCGAGAGGCCATATACTTGACAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000289235