Construct: shRNA TRCN0000037804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004157.1-464s1c1
DNA Barcode:
GCAGATTTAATAGACGAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAR2A (5576)
Gene Description:
protein kinase cAMP-dependent type II regulatory subunit alpha
Transcript:
RefSeq NM_004157.1 (NON-CURRENT)
Match location:
Position 464 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATTTAATAGACGAGTAT
Hairpin Sequence:
5'-CCGG-GCAGATTTAATAGACGAGTAT-CTCGAG-ATACTCGTCTATTAAATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATTTAATAGACGAGTATCTCGAGATACTCGTCTATTAAATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATTTAATAGACGAGTATCTCGAGATACTCGTCTATTAAATCTGC-3'

Other clones with same target sequence:

(none)