Construct: shRNA TRCN0000037837

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173515.1-757s1c1
DNA Barcode:
CACTGAATTATGGCCTCGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNKSR3 (154043)
Gene Description:
CNKSR family member 3
Transcript:
RefSeq NM_173515.1 (NON-CURRENT)
Match location:
Position 757 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037837)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTGAATTATGGCCTCGAAA
Hairpin Sequence:
5'-CCGG-CACTGAATTATGGCCTCGAAA-CTCGAG-TTTCGAGGCCATAATTCAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTGAATTATGGCCTCGAAACTCGAGTTTCGAGGCCATAATTCAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTGAATTATGGCCTCGAAACTCGAGTTTCGAGGCCATAATTCAGTG-3'

Other clones with same target sequence:

(none)