Construct: shRNA TRCN0000037852

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012474.3-542s1c1
DNA Barcode:
GAGGAGACAGTTACTGTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UCK2 (7371)
Gene Description:
uridine-cytidine kinase 2
Transcript:
RefSeq NM_012474.3 (NON-CURRENT)
Match location:
Position 542 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037852)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGACAGTTACTGTCTAT
Hairpin Sequence:
5'-CCGG-GAGGAGACAGTTACTGTCTAT-CTCGAG-ATAGACAGTAACTGTCTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGACAGTTACTGTCTATCTCGAGATAGACAGTAACTGTCTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGACAGTTACTGTCTATCTCGAGATAGACAGTAACTGTCTCCTC-3'

Other clones with same target sequence:

(none)