Construct: shRNA TRCN0000037865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020965.2-2984s1c1
DNA Barcode:
CCAGGAGTTATTCCTCATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGI3 (260425)
Gene Description:
membrane associated guanylate kinase, WW and PDZ domain containing 3
Transcript:
RefSeq NM_020965.2 (NON-CURRENT)
Match location:
Position 2984 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000037865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAGTTATTCCTCATAAA
Hairpin Sequence:
5'-CCGG-CCAGGAGTTATTCCTCATAAA-CTCGAG-TTTATGAGGAATAACTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAGTTATTCCTCATAAACTCGAGTTTATGAGGAATAACTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAGTTATTCCTCATAAACTCGAGTTTATGAGGAATAACTCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000333008