Construct: shRNA TRCN0000038036

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005380.3-256s1c1
DNA Barcode:
CAAGAACATCACCCAGATCGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NBL1 (4681)
Gene Description:
NBL1, DAN family BMP antagonist
Transcript:
RefSeq NM_005380.3 (NON-CURRENT)
Match location:
Position 256 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038036)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAACATCACCCAGATCGT
Hairpin Sequence:
5'-CCGG-CAAGAACATCACCCAGATCGT-CTCGAG-ACGATCTGGGTGATGTTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAACATCACCCAGATCGTCTCGAGACGATCTGGGTGATGTTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAACATCACCCAGATCGTCTCGAGACGATCTGGGTGATGTTCTTG-3'

Other clones with same target sequence:

(none)