Construct: shRNA TRCN0000038096

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015711.1-3114s1c1
DNA Barcode:
CGGAATCATCCTCCAGAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BICRA (29998)
Gene Description:
BRD4 interacting chromatin remodeling complex associated protein
Transcript:
RefSeq NM_015711.1 (NON-CURRENT)
Match location:
Position 3114 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038096)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAATCATCCTCCAGAACAA
Hairpin Sequence:
5'-CCGG-CGGAATCATCCTCCAGAACAA-CTCGAG-TTGTTCTGGAGGATGATTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAATCATCCTCCAGAACAACTCGAGTTGTTCTGGAGGATGATTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAATCATCCTCCAGAACAACTCGAGTTGTTCTGGAGGATGATTCCG-3'

Other clones with same target sequence:

(none)