Construct: shRNA TRCN0000038104

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_061871.7-9379s1c1
DNA Barcode:
GCCCTGCTTAACAAGAGCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAT3 (120114)
Gene Description:
FAT atypical cadherin 3
Transcript:
RefSeq XM_061871.7 (NON-CURRENT)
Match location:
Position 9379 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038104)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGCTTAACAAGAGCAAT
Hairpin Sequence:
5'-CCGG-GCCCTGCTTAACAAGAGCAAT-CTCGAG-ATTGCTCTTGTTAAGCAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGCTTAACAAGAGCAATCTCGAGATTGCTCTTGTTAAGCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGCTTAACAAGAGCAATCTCGAGATTGCTCTTGTTAAGCAGGGC-3'

Other clones with same target sequence:

(none)