Construct: shRNA TRCN0000038215

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006886.2-127s1c1
DNA Barcode:
CTACATCCGATACTCCCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP5F1E (514)
Gene Description:
ATP synthase F1 subunit epsilon
Transcript:
RefSeq NM_006886.2 (NON-CURRENT)
Match location:
Position 127 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038215)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACATCCGATACTCCCAGAT
Hairpin Sequence:
5'-CCGG-CTACATCCGATACTCCCAGAT-CTCGAG-ATCTGGGAGTATCGGATGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACATCCGATACTCCCAGATCTCGAGATCTGGGAGTATCGGATGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACATCCGATACTCCCAGATCTCGAGATCTGGGAGTATCGGATGTAG-3'

Other clones with same target sequence:

  1. TRCN0000291504