Construct: shRNA TRCN0000038227

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016609.2-279s1c1
DNA Barcode:
CTGATAGTGAAGCGGCAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC22A17 (51310)
Gene Description:
solute carrier family 22 member 17
Transcript:
RefSeq NM_016609.2 (NON-CURRENT)
Match location:
Position 279 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038227)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGATAGTGAAGCGGCAGATT
Hairpin Sequence:
5'-CCGG-CTGATAGTGAAGCGGCAGATT-CTCGAG-AATCTGCCGCTTCACTATCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGATAGTGAAGCGGCAGATTCTCGAGAATCTGCCGCTTCACTATCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGATAGTGAAGCGGCAGATTCTCGAGAATCTGCCGCTTCACTATCAG-3'

Other clones with same target sequence:

(none)