Construct: shRNA TRCN0000038239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030958.1-1705s1c1
DNA Barcode:
CCCAATGTTTACTTTCCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLCO5A1 (81796)
Gene Description:
solute carrier organic anion transporter family member 5A1
Transcript:
RefSeq NM_030958.1 (NON-CURRENT)
Match location:
Position 1705 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATGTTTACTTTCCCAAA
Hairpin Sequence:
5'-CCGG-CCCAATGTTTACTTTCCCAAA-CTCGAG-TTTGGGAAAGTAAACATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATGTTTACTTTCCCAAACTCGAGTTTGGGAAAGTAAACATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATGTTTACTTTCCCAAACTCGAGTTTGGGAAAGTAAACATTGGG-3'

Other clones with same target sequence:

(none)