Construct: shRNA TRCN0000038279

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001044.2-3236s1c1
DNA Barcode:
GCCACATCAATAACAACAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC6A3 (6531)
Gene Description:
solute carrier family 6 member 3
Transcript:
RefSeq NM_001044.2 (NON-CURRENT)
Match location:
Position 3236 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038279)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACATCAATAACAACAGTT
Hairpin Sequence:
5'-CCGG-GCCACATCAATAACAACAGTT-CTCGAG-AACTGTTGTTATTGATGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACATCAATAACAACAGTTCTCGAGAACTGTTGTTATTGATGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACATCAATAACAACAGTTCTCGAGAACTGTTGTTATTGATGTGGC-3'

Other clones with same target sequence:

(none)