Construct: shRNA TRCN0000038314

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032513.2-736s1c1
DNA Barcode:
CCATGTGATCGGCGACTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC30A2 (7780)
Gene Description:
solute carrier family 30 member 2
Transcript:
RefSeq NM_032513.2 (NON-CURRENT)
Match location:
Position 736 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038314)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTGATCGGCGACTTTAT
Hairpin Sequence:
5'-CCGG-CCATGTGATCGGCGACTTTAT-CTCGAG-ATAAAGTCGCCGATCACATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTGATCGGCGACTTTATCTCGAGATAAAGTCGCCGATCACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTGATCGGCGACTTTATCTCGAGATAAAGTCGCCGATCACATGG-3'

Other clones with same target sequence:

(none)