Construct: shRNA TRCN0000038348

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003459.3-259s1c1
DNA Barcode:
CGTTTGAAGAGTCTCTTCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC30A3 (7781)
Gene Description:
solute carrier family 30 member 3
Transcript:
RefSeq NM_003459.3 (NON-CURRENT)
Match location:
Position 259 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038348)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTTGAAGAGTCTCTTCACA
Hairpin Sequence:
5'-CCGG-CGTTTGAAGAGTCTCTTCACA-CTCGAG-TGTGAAGAGACTCTTCAAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTTGAAGAGTCTCTTCACACTCGAGTGTGAAGAGACTCTTCAAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTTGAAGAGTCTCTTCACACTCGAGTGTGAAGAGACTCTTCAAACG-3'

Other clones with same target sequence:

(none)