Construct: shRNA TRCN0000038355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020208.2-1594s1c1
DNA Barcode:
CTGCTGATTGTCAGCCTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC6A20 (54716)
Gene Description:
solute carrier family 6 member 20
Transcript:
RefSeq NM_020208.2 (NON-CURRENT)
Match location:
Position 1594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTGATTGTCAGCCTCTTT
Hairpin Sequence:
5'-CCGG-CTGCTGATTGTCAGCCTCTTT-CTCGAG-AAAGAGGCTGACAATCAGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTGATTGTCAGCCTCTTTCTCGAGAAAGAGGCTGACAATCAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTGATTGTCAGCCTCTTTCTCGAGAAAGAGGCTGACAATCAGCAG-3'

Other clones with same target sequence:

(none)