Construct: shRNA TRCN0000038377

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013309.3-339s1c1
DNA Barcode:
CCTCAAATCTATGCTAAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC30A4 (7782)
Gene Description:
solute carrier family 30 member 4
Transcript:
RefSeq NM_013309.3 (NON-CURRENT)
Match location:
Position 339 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038377)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAAATCTATGCTAAGGAA
Hairpin Sequence:
5'-CCGG-CCTCAAATCTATGCTAAGGAA-CTCGAG-TTCCTTAGCATAGATTTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAAATCTATGCTAAGGAACTCGAGTTCCTTAGCATAGATTTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAAATCTATGCTAAGGAACTCGAGTTCCTTAGCATAGATTTGAGG-3'

Other clones with same target sequence:

(none)