Construct: shRNA TRCN0000038384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130463.2-409s1c1
DNA Barcode:
GCACAGATGGAGGTGGAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V1G2 (534)
Gene Description:
ATPase H+ transporting V1 subunit G2
Transcript:
RefSeq NM_130463.2 (NON-CURRENT)
Match location:
Position 409 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGATGGAGGTGGAGCAA
Hairpin Sequence:
5'-CCGG-GCACAGATGGAGGTGGAGCAA-CTCGAG-TTGCTCCACCTCCATCTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGATGGAGGTGGAGCAACTCGAGTTGCTCCACCTCCATCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGATGGAGGTGGAGCAACTCGAGTTGCTCCACCTCCATCTGTGC-3'

Other clones with same target sequence:

(none)