Construct: shRNA TRCN0000038417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015684.1-967s1c1
DNA Barcode:
CGACTCTTGTATCATGAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DMAC2L (27109)
Gene Description:
distal membrane arm assembly complex 2 like
Transcript:
RefSeq NM_015684.1 (NON-CURRENT)
Match location:
Position 967 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTCTTGTATCATGAGCAT
Hairpin Sequence:
5'-CCGG-CGACTCTTGTATCATGAGCAT-CTCGAG-ATGCTCATGATACAAGAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTCTTGTATCATGAGCATCTCGAGATGCTCATGATACAAGAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTCTTGTATCATGAGCATCTCGAGATGCTCATGATACAAGAGTCG-3'

Other clones with same target sequence:

(none)