Construct: shRNA TRCN0000038429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005177.2-955s1c1
DNA Barcode:
CCAGGATTGATGATCTCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V0A1 (535)
Gene Description:
ATPase H+ transporting V0 subunit a1
Transcript:
RefSeq NM_005177.2 (NON-CURRENT)
Match location:
Position 955 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGATTGATGATCTCCAAA
Hairpin Sequence:
5'-CCGG-CCAGGATTGATGATCTCCAAA-CTCGAG-TTTGGAGATCATCAATCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGATTGATGATCTCCAAACTCGAGTTTGGAGATCATCAATCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGATTGATGATCTCCAAACTCGAGTTTGGAGATCATCAATCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000307276