Construct: shRNA TRCN0000038451

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015359.1-278s1c1
DNA Barcode:
GCAGGATCTAATACATCGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC39A14 (23516)
Gene Description:
solute carrier family 39 member 14
Transcript:
RefSeq NM_015359.1 (NON-CURRENT)
Match location:
Position 278 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038451)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGATCTAATACATCGGTA
Hairpin Sequence:
5'-CCGG-GCAGGATCTAATACATCGGTA-CTCGAG-TACCGATGTATTAGATCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGATCTAATACATCGGTACTCGAGTACCGATGTATTAGATCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGATCTAATACATCGGTACTCGAGTACCGATGTATTAGATCCTGC-3'

Other clones with same target sequence:

(none)