Construct: shRNA TRCN0000038470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004320.2-1682s1c1
DNA Barcode:
CGCTGTAACTATGTGCGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP2A1 (487)
Gene Description:
ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1
Transcript:
RefSeq NM_004320.2 (NON-CURRENT)
Match location:
Position 1682 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGTAACTATGTGCGAGTT
Hairpin Sequence:
5'-CCGG-CGCTGTAACTATGTGCGAGTT-CTCGAG-AACTCGCACATAGTTACAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGTAACTATGTGCGAGTTCTCGAGAACTCGCACATAGTTACAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGTAACTATGTGCGAGTTCTCGAGAACTCGCACATAGTTACAGCG-3'

Other clones with same target sequence:

(none)