Construct: shRNA TRCN0000038480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012319.2-1689s1c1
DNA Barcode:
CCACAGGAAGTCTACAATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC39A6 (25800)
Gene Description:
solute carrier family 39 member 6
Transcript:
RefSeq NM_012319.2 (NON-CURRENT)
Match location:
Position 1689 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGGAAGTCTACAATGAA
Hairpin Sequence:
5'-CCGG-CCACAGGAAGTCTACAATGAA-CTCGAG-TTCATTGTAGACTTCCTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGGAAGTCTACAATGAACTCGAGTTCATTGTAGACTTCCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGGAAGTCTACAATGAACTCGAGTTCATTGTAGACTTCCTGTGG-3'

Other clones with same target sequence:

(none)