Construct: shRNA TRCN0000038514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371280.1-1344s1c1
DNA Barcode:
CGGAAACTACTTTGTCACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC6A17 (388662)
Gene Description:
solute carrier family 6 member 17
Transcript:
RefSeq XM_371280.1 (NON-CURRENT)
Match location:
Position 1344 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAACTACTTTGTCACCAT
Hairpin Sequence:
5'-CCGG-CGGAAACTACTTTGTCACCAT-CTCGAG-ATGGTGACAAAGTAGTTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAACTACTTTGTCACCATCTCGAGATGGTGACAAAGTAGTTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAACTACTTTGTCACCATCTCGAGATGGTGACAAAGTAGTTTCCG-3'

Other clones with same target sequence:

(none)