Construct: shRNA TRCN0000038523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003945.2-135s1c1
DNA Barcode:
CGGCTTCTTGGTGCCTTGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V0E1 (8992)
Gene Description:
ATPase H+ transporting V0 subunit e1
Transcript:
RefSeq NM_003945.2 (NON-CURRENT)
Match location:
Position 135 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTTCTTGGTGCCTTGGTT
Hairpin Sequence:
5'-CCGG-CGGCTTCTTGGTGCCTTGGTT-CTCGAG-AACCAAGGCACCAAGAAGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTTCTTGGTGCCTTGGTTCTCGAGAACCAAGGCACCAAGAAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTTCTTGGTGCCTTGGTTCTCGAGAACCAAGGCACCAAGAAGCCG-3'

Other clones with same target sequence:

(none)