Construct: shRNA TRCN0000038536

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018593.3-689s1c1
DNA Barcode:
CCTTTGCATACCAGCCTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC16A10 (117247)
Gene Description:
solute carrier family 16 member 10
Transcript:
RefSeq NM_018593.3 (NON-CURRENT)
Match location:
Position 689 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038536)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGCATACCAGCCTTCAT
Hairpin Sequence:
5'-CCGG-CCTTTGCATACCAGCCTTCAT-CTCGAG-ATGAAGGCTGGTATGCAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGCATACCAGCCTTCATCTCGAGATGAAGGCTGGTATGCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGCATACCAGCCTTCATCTCGAGATGAAGGCTGGTATGCAAAGG-3'

Other clones with same target sequence:

(none)