Construct: shRNA TRCN0000038542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144564.3-417s1c1
DNA Barcode:
CGGCCACATCAGCACCGACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC39A3 (29985)
Gene Description:
solute carrier family 39 member 3
Transcript:
RefSeq NM_144564.3 (NON-CURRENT)
Match location:
Position 417 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCCACATCAGCACCGACTA
Hairpin Sequence:
5'-CCGG-CGGCCACATCAGCACCGACTA-CTCGAG-TAGTCGGTGCTGATGTGGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCCACATCAGCACCGACTACTCGAGTAGTCGGTGCTGATGTGGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCCACATCAGCACCGACTACTCGAGTAGTCGGTGCTGATGTGGCCG-3'

Other clones with same target sequence:

(none)