Construct: shRNA TRCN0000038634

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006019.2-253s1c1
DNA Barcode:
AGACGCTTTGTGGTTGATGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCIRG1 (10312)
Gene Description:
T cell immune regulator 1, ATPase H+ transporting V0 subunit a3
Transcript:
RefSeq NM_006019.2 (NON-CURRENT)
Match location:
Position 253 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038634)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACGCTTTGTGGTTGATGTT
Hairpin Sequence:
5'-CCGG-AGACGCTTTGTGGTTGATGTT-CTCGAG-AACATCAACCACAAAGCGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACGCTTTGTGGTTGATGTTCTCGAGAACATCAACCACAAAGCGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACGCTTTGTGGTTGATGTTCTCGAGAACATCAACCACAAAGCGTCT-3'

Other clones with same target sequence:

  1. TRCN0000289222