Construct: shRNA TRCN0000038640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003038.2-748s1c1
DNA Barcode:
CCGTACGTATGCAACCGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC1A4 (6509)
Gene Description:
solute carrier family 1 member 4
Transcript:
RefSeq NM_003038.2 (NON-CURRENT)
Match location:
Position 748 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTACGTATGCAACCGATTA
Hairpin Sequence:
5'-CCGG-CCGTACGTATGCAACCGATTA-CTCGAG-TAATCGGTTGCATACGTACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTACGTATGCAACCGATTACTCGAGTAATCGGTTGCATACGTACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTACGTATGCAACCGATTACTCGAGTAATCGGTTGCATACGTACGG-3'

Other clones with same target sequence:

(none)