Construct: shRNA TRCN0000038739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002546.2-324s1c1
DNA Barcode:
CCTCCAAAGTACCTTCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFRSF11B (4982)
Gene Description:
TNF receptor superfamily member 11b
Transcript:
RefSeq NM_002546.2 (NON-CURRENT)
Match location:
Position 324 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCAAAGTACCTTCATTAT
Hairpin Sequence:
5'-CCGG-CCTCCAAAGTACCTTCATTAT-CTCGAG-ATAATGAAGGTACTTTGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCAAAGTACCTTCATTATCTCGAGATAATGAAGGTACTTTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCAAAGTACCTTCATTATCTCGAGATAATGAAGGTACTTTGGAGG-3'

Other clones with same target sequence:

(none)