Construct: shRNA TRCN0000038757

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013240.2-635s1c1
DNA Barcode:
AGAAACTCTTTCAGTCCTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
N6AMT1 (29104)
Gene Description:
N-6 adenine-specific DNA methyltransferase 1
Transcript:
RefSeq NM_013240.2 (NON-CURRENT)
Match location:
Position 635 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038757)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAACTCTTTCAGTCCTCAA
Hairpin Sequence:
5'-CCGG-AGAAACTCTTTCAGTCCTCAA-CTCGAG-TTGAGGACTGAAAGAGTTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAACTCTTTCAGTCCTCAACTCGAGTTGAGGACTGAAAGAGTTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAACTCTTTCAGTCCTCAACTCGAGTTGAGGACTGAAAGAGTTTCT-3'

Other clones with same target sequence:

(none)