Construct: shRNA TRCN0000038779

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030934.3-783s1c1
DNA Barcode:
GCCCATTATCATTGTATCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRMT1L (81627)
Gene Description:
tRNA methyltransferase 1 like
Transcript:
RefSeq NM_030934.3 (NON-CURRENT)
Match location:
Position 783 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038779)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATTATCATTGTATCATT
Hairpin Sequence:
5'-CCGG-GCCCATTATCATTGTATCATT-CTCGAG-AATGATACAATGATAATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATTATCATTGTATCATTCTCGAGAATGATACAATGATAATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATTATCATTGTATCATTCTCGAGAATGATACAATGATAATGGGC-3'

Other clones with same target sequence:

(none)