Construct: shRNA TRCN0000038780

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030934.3-1019s1c1
DNA Barcode:
GCAGCTAATATTCTGTACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRMT1L (81627)
Gene Description:
tRNA methyltransferase 1 like
Transcript:
RefSeq NM_030934.3 (NON-CURRENT)
Match location:
Position 1019 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038780)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTAATATTCTGTACATT
Hairpin Sequence:
5'-CCGG-GCAGCTAATATTCTGTACATT-CTCGAG-AATGTACAGAATATTAGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTAATATTCTGTACATTCTCGAGAATGTACAGAATATTAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTAATATTCTGTACATTCTCGAGAATGTACAGAATATTAGCTGC-3'

Other clones with same target sequence:

(none)