Construct: shRNA TRCN0000038819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032663.2-1860s1c1
DNA Barcode:
CTGTTGTGTTAAGAAAGCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP30 (84749)
Gene Description:
ubiquitin specific peptidase 30
Transcript:
RefSeq NM_032663.2 (NON-CURRENT)
Match location:
Position 1860 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTTGTGTTAAGAAAGCATT
Hairpin Sequence:
5'-CCGG-CTGTTGTGTTAAGAAAGCATT-CTCGAG-AATGCTTTCTTAACACAACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTTGTGTTAAGAAAGCATTCTCGAGAATGCTTTCTTAACACAACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTTGTGTTAAGAAAGCATTCTCGAGAATGCTTTCTTAACACAACAG-3'

Other clones with same target sequence:

(none)