Construct: shRNA TRCN0000038915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024513.1-257s1c1
DNA Barcode:
GCTGTGACAGAACTAAGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FYCO1 (79443)
Gene Description:
FYVE and coiled-coil domain autophagy adaptor 1
Transcript:
RefSeq NM_024513.1 (NON-CURRENT)
Match location:
Position 257 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGACAGAACTAAGCAAA
Hairpin Sequence:
5'-CCGG-GCTGTGACAGAACTAAGCAAA-CTCGAG-TTTGCTTAGTTCTGTCACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGACAGAACTAAGCAAACTCGAGTTTGCTTAGTTCTGTCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGACAGAACTAAGCAAACTCGAGTTTGCTTAGTTCTGTCACAGC-3'

Other clones with same target sequence:

(none)