Construct: shRNA TRCN0000038951

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001933.1-876s1c1
DNA Barcode:
GCAAGTGTGTGTGCAACAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LHX8 (431707)
Gene Description:
LIM homeobox 8
Transcript:
RefSeq NM_001001933.1 (CURRENT)
Match location:
Position 876 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000038951)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGTGTGTGTGCAACAGTT
Hairpin Sequence:
5'-CCGG-GCAAGTGTGTGTGCAACAGTT-CTCGAG-AACTGTTGCACACACACTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGTGTGTGTGCAACAGTTCTCGAGAACTGTTGCACACACACTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGTGTGTGTGCAACAGTTCTCGAGAACTGTTGCACACACACTTGC-3'

Other clones with same target sequence:

(none)