Construct: shRNA TRCN0000039004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021199.1-1306s1c1
DNA Barcode:
GCGCCTTTCCATGTATCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SQOR (58472)
Gene Description:
sulfide quinone oxidoreductase
Transcript:
RefSeq NM_021199.1 (NON-CURRENT)
Match location:
Position 1306 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCCTTTCCATGTATCTCAT
Hairpin Sequence:
5'-CCGG-GCGCCTTTCCATGTATCTCAT-CTCGAG-ATGAGATACATGGAAAGGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCCTTTCCATGTATCTCATCTCGAGATGAGATACATGGAAAGGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCCTTTCCATGTATCTCATCTCGAGATGAGATACATGGAAAGGCGC-3'

Other clones with same target sequence:

(none)