Construct: shRNA TRCN0000039009

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016576.2-1128s1c1
DNA Barcode:
CTATGGAATGAGTTCTGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GMPR2 (51292)
Gene Description:
guanosine monophosphate reductase 2
Transcript:
RefSeq NM_016576.2 (NON-CURRENT)
Match location:
Position 1128 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039009)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGGAATGAGTTCTGAAAT
Hairpin Sequence:
5'-CCGG-CTATGGAATGAGTTCTGAAAT-CTCGAG-ATTTCAGAACTCATTCCATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGGAATGAGTTCTGAAATCTCGAGATTTCAGAACTCATTCCATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGGAATGAGTTCTGAAATCTCGAGATTTCAGAACTCATTCCATAG-3'

Other clones with same target sequence:

  1. TRCN0000286350