Construct: shRNA TRCN0000039023

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007794.1-2338s1c1
DNA Barcode:
GCCATCATTCAGGTCGAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctcf (13018)
Gene Description:
CCCTC-binding factor
Transcript:
RefSeq NM_007794.1 (NON-CURRENT)
Match location:
Position 2338 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039023)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCATTCAGGTCGAAGAT
Hairpin Sequence:
5'-CCGG-GCCATCATTCAGGTCGAAGAT-CTCGAG-ATCTTCGACCTGAATGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCATTCAGGTCGAAGATCTCGAGATCTTCGACCTGAATGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCATTCAGGTCGAAGATCTCGAGATCTTCGACCTGAATGATGGC-3'

Other clones with same target sequence:

(none)