Construct: shRNA TRCN0000039038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007872.2-2505s1c1
DNA Barcode:
CCCGTGATGATTGACGCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnmt3a (13435)
Gene Description:
DNA methyltransferase 3A
Transcript:
RefSeq NM_007872.2 (NON-CURRENT)
Match location:
Position 2505 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTGATGATTGACGCCAAA
Hairpin Sequence:
5'-CCGG-CCCGTGATGATTGACGCCAAA-CTCGAG-TTTGGCGTCAATCATCACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTGATGATTGACGCCAAACTCGAGTTTGGCGTCAATCATCACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTGATGATTGACGCCAAACTCGAGTTTGGCGTCAATCATCACGGG-3'

Other clones with same target sequence:

(none)