Construct: shRNA TRCN0000039052

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010610.1-1350s1c1
DNA Barcode:
CCACCCAAAGATCAGGATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnma1 (16531)
Gene Description:
potassium large conductance calcium-activated channel, subfamily M, alpha member 1
Transcript:
RefSeq NM_010610.1 (NON-CURRENT)
Match location:
Position 1350 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039052)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCCAAAGATCAGGATCAT
Hairpin Sequence:
5'-CCGG-CCACCCAAAGATCAGGATCAT-CTCGAG-ATGATCCTGATCTTTGGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCCAAAGATCAGGATCATCTCGAGATGATCCTGATCTTTGGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCCAAAGATCAGGATCATCTCGAGATGATCCTGATCTTTGGGTGG-3'

Other clones with same target sequence:

(none)