Construct: shRNA TRCN0000039053

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010610.1-376s1c1
DNA Barcode:
GCCCTCGTAATATACTTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnma1 (16531)
Gene Description:
potassium large conductance calcium-activated channel, subfamily M, alpha member 1
Transcript:
RefSeq NM_010610.1 (NON-CURRENT)
Match location:
Position 376 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039053)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCGTAATATACTTCATA
Hairpin Sequence:
5'-CCGG-GCCCTCGTAATATACTTCATA-CTCGAG-TATGAAGTATATTACGAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCGTAATATACTTCATACTCGAGTATGAAGTATATTACGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCGTAATATACTTCATACTCGAGTATGAAGTATATTACGAGGGC-3'

Other clones with same target sequence:

(none)