Construct: shRNA TRCN0000039091

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009122.1-1842s1c1
DNA Barcode:
CCTGTCGATGATCCGAAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Satb1 (20230)
Gene Description:
special AT-rich sequence binding protein 1
Transcript:
RefSeq NM_009122.1 (NON-CURRENT)
Match location:
Position 1842 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039091)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCGATGATCCGAAGATT
Hairpin Sequence:
5'-CCGG-CCTGTCGATGATCCGAAGATT-CTCGAG-AATCTTCGGATCATCGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCGATGATCCGAAGATTCTCGAGAATCTTCGGATCATCGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCGATGATCCGAAGATTCTCGAGAATCTTCGGATCATCGACAGG-3'

Other clones with same target sequence:

(none)