Construct: shRNA TRCN0000039143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026366.1-461s1c1
DNA Barcode:
AGAGGTAGGAAGTCGTGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
N6amt1 (67768)
Gene Description:
N-6 adenine-specific DNA methyltransferase 1 (putative)
Transcript:
RefSeq NM_026366.1 (NON-CURRENT)
Match location:
Position 461 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGTAGGAAGTCGTGGAAT
Hairpin Sequence:
5'-CCGG-AGAGGTAGGAAGTCGTGGAAT-CTCGAG-ATTCCACGACTTCCTACCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGTAGGAAGTCGTGGAATCTCGAGATTCCACGACTTCCTACCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGTAGGAAGTCGTGGAATCTCGAGATTCCACGACTTCCTACCTCT-3'

Other clones with same target sequence:

(none)