Construct: shRNA TRCN0000039216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177767.3-194s1c1
DNA Barcode:
CCAAACTTCATCCAAAGCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ogfod1 (270086)
Gene Description:
2-oxoglutarate and iron-dependent oxygenase domain containing 1
Transcript:
RefSeq NM_177767.3 (NON-CURRENT)
Match location:
Position 194 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACTTCATCCAAAGCCAA
Hairpin Sequence:
5'-CCGG-CCAAACTTCATCCAAAGCCAA-CTCGAG-TTGGCTTTGGATGAAGTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACTTCATCCAAAGCCAACTCGAGTTGGCTTTGGATGAAGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACTTCATCCAAAGCCAACTCGAGTTGGCTTTGGATGAAGTTTGG-3'

Other clones with same target sequence:

(none)