Construct: shRNA TRCN0000039259

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054081.1-1107s1c1
DNA Barcode:
GCAGGTTTATATTCCCAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mta1 (116870)
Gene Description:
metastasis associated 1
Transcript:
RefSeq NM_054081.1 (NON-CURRENT)
Match location:
Position 1107 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039259)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTTTATATTCCCAACTA
Hairpin Sequence:
5'-CCGG-GCAGGTTTATATTCCCAACTA-CTCGAG-TAGTTGGGAATATAAACCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTTTATATTCCCAACTACTCGAGTAGTTGGGAATATAAACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTTTATATTCCCAACTACTCGAGTAGTTGGGAATATAAACCTGC-3'

Other clones with same target sequence:

  1. TRCN0000334112