Construct: shRNA TRCN0000039309

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028811.1-1280s1c1
DNA Barcode:
CGGAGAGATTATGTTGCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elp3 (74195)
Gene Description:
elongator acetyltransferase complex subunit 3
Transcript:
RefSeq NM_028811.1 (NON-CURRENT)
Match location:
Position 1280 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039309)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGAGATTATGTTGCCAAT
Hairpin Sequence:
5'-CCGG-CGGAGAGATTATGTTGCCAAT-CTCGAG-ATTGGCAACATAATCTCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGAGATTATGTTGCCAATCTCGAGATTGGCAACATAATCTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGAGATTATGTTGCCAATCTCGAGATTGGCAACATAATCTCTCCG-3'

Other clones with same target sequence:

  1. TRCN0000326977