Construct: shRNA TRCN0000039333

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022433.1-1006s1c1
DNA Barcode:
CACAAGAACTGCTGGATCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sirt3 (64384)
Gene Description:
sirtuin 3
Transcript:
RefSeq NM_022433.1 (NON-CURRENT)
Match location:
Position 1006 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039333)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAAGAACTGCTGGATCTTA
Hairpin Sequence:
5'-CCGG-CACAAGAACTGCTGGATCTTA-CTCGAG-TAAGATCCAGCAGTTCTTGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAAGAACTGCTGGATCTTACTCGAGTAAGATCCAGCAGTTCTTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAAGAACTGCTGGATCTTACTCGAGTAAGATCCAGCAGTTCTTGTG-3'

Other clones with same target sequence:

  1. TRCN0000332648