Construct: shRNA TRCN0000039343
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_017479.1-2772s1c1
- DNA Barcode:
- CCACAGTCAGTTTCCATTAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Kat6b (54169)
- Gene Description:
- K(lysine) acetyltransferase 6B
- Transcript:
- RefSeq NM_017479.1 (NON-CURRENT)
- Match location:
- Position 2772 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000039343)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCACAGTCAGTTTCCATTAAA
- Hairpin Sequence:
-
5'-CCGG-CCACAGTCAGTTTCCATTAAA-CTCGAG-TTTAATGGAAACTGACTGTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCACAGTCAGTTTCCATTAAACTCGAGTTTAATGGAAACTGACTGTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCACAGTCAGTTTCCATTAAACTCGAGTTTAATGGAAACTGACTGTGG-3'
Other clones with same target sequence:
-
TRCN0000287544
